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2 edition of nitric oxide-cGMP system in the human pregnant myometrial cells and its regulation by CRH found in the catalog.

nitric oxide-cGMP system in the human pregnant myometrial cells and its regulation by CRH

Eleni Angelidou

nitric oxide-cGMP system in the human pregnant myometrial cells and its regulation by CRH

by Eleni Angelidou

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  • 14 Currently reading

Published by typescript in [s.l.] .
Written in English


Edition Notes

Thesis (M.Sc.) - University of Warwick, 2000.

StatementEleni Angelidou.
The Physical Object
Paginationxi, 79p.
Number of Pages79
ID Numbers
Open LibraryOL19603403M

Conversely, nitric oxide (NO), originally described as a non-prostaglandin, endothelium-derived relaxing factor (EDRF), in addition to its potent vasodilator action, has emerged as an important vasculo-protective agent through its ability to exert anti-hypertrophic, anti-proliferative and anti-apoptotic responses in the cardiovascular system [3.   Parturition requires that myometrial smooth muscle undergo a phenotypic transition, remaining quiescent for the majority of gestation and then transforming to a tissue capable of generating forceful, coordinated contractions to expel the fetus and the placenta [1, 2, 3].Characterizing the regulation of key myometrial genes is essential to understanding normal human birth, as well as .

In cultured human pregnant myometrial cells, urocortin but not CRH was able to induce MAPK phosphorylation and activation, suggesting that in the human myometrium these .   Nitric Oxide‐cGMP‐PKG Pathway Acts on Orai1 to Inhibit the Hypertrophy of Human Embryonic Stem Cell‐Derived Cardiomyocytes Y. Wang Li Ka Shing Institute of Health Sciences and School of Biomedical Sciences, Faculty of Medicine, the Chinese University of Hong Kong, Hong Kong, People's Republic of China.

  Nitric oxide‐cGMP signaling regulates axonal elongation during optic nerve regeneration in the goldfish in vitro and in vivo Yoshiki Koriyama Department of Molecular Neurobiology and Division of Laboratory Sciences, Graduate School of Medicine, Kanazawa University, Kanazawa, Japan.   Nitric oxide is best known as an air pollutant produced by vehicle emissions and power plants but for pregnant women it is a crucial compound required to avoid hypertension and pre-eclampsia. Now.


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Nitric oxide-cGMP system in the human pregnant myometrial cells and its regulation by CRH by Eleni Angelidou Download PDF EPUB FB2

Although the sGC in human myometrium has yet to be characterized, it is possible that such an enzyme plays a passive role in the regulation of myometrial relaxation during pregnancy. Evidence for this possibility stems from the ability of OT to increase intracellular calcium while simultaneously decreasing CysNO-induced cGMP by: 4.

tions between CRH and the NO cGMP system in the human pregnant myometrium by using primary human pregnant myo-metrial cell cultures. Materials and Methods Experimental Subjects and Preparation of Myometrial Cell Cultures.

Myometrial tissue was obtained from the lower segment of the uterus of women undergoing elective caesarean section for non-Cited by:   CRH-Induced G-Protein Activation in Human Pregnant Myometrial Cells.

CRH, acting via adenylate cyclase, can activate PKA. Because the CRH effect on myometrial NOS expression was not affected by PKA inhibition, we investigated whether CRH may act via alternative pathways by characterizing the G-protein subtypes activated by CRH in myometrial by: The vascular nitric oxide‐cGMP pathway is disturbed in DM, involving oxidative stress and increased PDE expression.

In cultured aortic VSMCs of hyperglycaemic rats, cinaciguat efficiently reduced pro‐fibrotic TGF‐β1 and matrix metalloproteinase dysregulation, and restored decreased myocardial cGMP levels in vivo to the level of non Cited by: 3.

Nitric Oxide‐cGMP‐PKG Pathway Acts on Orai1 to Inhibit the Hypertrophy of Human Embryonic Stem Cell‐Derived Cardiomyocytes Y.

Wang Li Ka Shing Institute of Health Sciences and School of Biomedical Sciences, Faculty of Medicine, the Chinese University of Hong Kong, Hong Kong, People's Republic of ChinaCited by: The role of the nitric oxide (NO)‐cGMP pathway in the cholinergic modulation of cardiac pacemaking is controversial.

Whilst some groups have shown that inhibition of endothelial nitric oxide synthase (eNOS), either pharmacologically (Han et al.; Balligand, ) or via gene knockout (Han et al.

b), abolishes the reduction in L‐type calcium current (I Ca,L) in response to. In the myometrial smooth-muscle cells during pregnancy, the effects of NO are mediated by activation of the NO-sensitive soluble form of guanylyl cyclase (sGC), the enzyme responsible for cyclic-guanosine-monophosphate (cGMP) generation and protein-kinase-G (PKG) activation, and by cGMP-independent intracellular effects that involve signalling molecules such as Ca 2+-activated and ATP-activated K +.

The purpose of this study was to identify cell types in human myometrium that contain inducible nitric oxide synthase (iNOS), and to examine changes in its expression during pregnancy and labor. We found that iNOS is expressed in smooth muscle cells of pregnant myometrium.

Expression of iNOS was highest in myometrium of preterm not-in-labor patients. The binding of ET-1 to the ETAs on human myometrial cells results in a rapid increase of cytosolic free calcium (Ca 2+) concentration by mechanisms involving both the mobilization of Ca 2+ from intracellular stores, as well as Ca 2+ influx through Ca 2+ channels [3,4], with a strong vasoconstrictor effect as outcome.

These studies indicate that an L-arginine­nitric oxide-cGMP system may contribute to the quies­cent state ofuterus during pregnancy. Conversely, it is possible that the nitric oxide synthetic pathway and the relaxation response may be subdued in uterine tissue during labor and pave the way for an increased con­tractile activity.

The nitric-oxide (NO) signalling cascade is important for placental function, in particular for the development of the vascular network and for maintaining vascular tone. This pathway seems to be regulated by multiple hormonal signals. These results indicate that in the human fetal-placental circulation, CRH causes a vasodilatory response via a nitric oxide-/cGMP-dependent pathway.

CRH may play a role in the control of vascular. The Endothelium-Dependent Nitric Oxide–cGMP Pathway on disorders of the circulation system and 3) the epigenetic regulation of NO-sGC pathway components in the cardiovascular system. This increase in cGMP concentration is apparently due to NO generation, because (pre)incubation of the cells with the competitive NO synthase inhibitor L-NAME ( μmol/L) prevented the thrombin-induced increase in these cells (± pmol cGMP/×10 5 cells; Pcells).

cGMP was not significantly. In this study, the expression, localisation and regulation of nitric oxide synthase (NOS) isoforms have been examined in human pregnant myometrium and cultured human myometrial smooth muscle cells, by immunoblotting, immunohistochemistry and reverse transcription-polymerase chain reaction.

In human endometrium, both epithelial and stroma cells produce corticotropin-releasing hormone (CRH). Both types of cells also possess specific CRH-bi. During pregnancy, CRH and CRH-related peptides appear to regulate the fetoplacental circulation via activation of the nitric oxide (NO)/cGMP pathway.

The nitric oxide–cGMP signaling pathway plays a significant role in tolerance to the analgesic effect of morphine. Ercan Ozdemir, a Ihsan Bagcivan, b Nedim Durmus, b Ahmet Altun, b Sinan Gursoy c. a Department of Physiology, Cumhuriyet University School of Medicine, Sivas, Turkey. E. Aggelidou, E.W.

Hillhouse, D.K. GrammatopoulosUp-regulation of nitric oxide synthase and modulation of the guanylate cyclase activity by corticotropin-releasing hormone but not urocortin II or urocortin III in cultured human pregnant myometrial cells. Regulatory functions of nitric oxide (NO•) that bypass the second messenger cGMP are incompletely understood.

Here, cGMP-independent effects of NO• on gene expression were globally examined in U cells, a human monoblastoid line that constitutively lacks soluble guanylate cyclase.

Differentiated U cells (>80% in G0/G1) were exposed to S-nitrosoglutathione, a NO• donor, or. Pregnancy; Nitric oxide Pregnancy Warnings. This drug is not indicated for use in adult patients.

AU TGA pregnancy category: B2 US FDA pregnancy category: C. Animal studies have not been conducted. There are no controlled data in human pregnancy.Ferid Murad, M.D., Ph.D. As I completed my training at the National Institutes of Health in to accept a faculty position at the University of Virginia, I decided to move most of my research.The inter- and intracellular regulator nitric oxide (NO) has been suggested to play a role in the modulation of cellular excitability, but the mechanism(s) by which this occurs remain unclear.

Using the kidney as a model system, we report here evidence that NO, produced in response to various hormones and cytokines, can effect long-term alterations in the activity of the membrane sodium pump.